Supporting the health of livestock through the fusion of traditional Japanese wisdom and advanced technology.

  • Strengthen gut health and immunity
  • Improve nutrient absorption and performance
  • Reduce odors and digestive stress
  • Minimize antibiotic use and drug residues
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Proactively Enhance Livestock Health and Performance—Naturally

A Smarter, More Sustainable Approach to Animal Care

Even on well-managed farms, livestock face routine stress from environmental conditions, dietary changes, and daily operations. These stressors can quietly impact growth, feed efficiency, immunity, and productivity.

Rather than waiting for issues to arise, support animal health from the inside out with MAKITO’s Mugwort Fermented Lactic Acid Bacteria—a 100% plant-based probiotic developed using patented Japanese fermentation technology.

This natural supplement helps you:

  • Strengthen gut health and immunity
  • Improve nutrient absorption and performance
  • Reduce odors and digestive stress
  • Minimize antibiotic use and drug residues

Proven effective in poultry, pigs, cattle, and sheep, it’s a proactive, sustainable solution to help your animals thrive—every day.

These challenges can be addressed—naturally and effectively.

  • Feed Costs
    Rising Mortality Rates and Escalating Feed Costs
  • Reduced Productivity
    Reduced Productivity Caused by Infections Like Colibacillosis
  • Antimicrobial Resistance
    The Growing Challenge of Antimicrobial Resistance

Optimizing the Intestinal Flora of Livestock 100% Natural Probiotic:
"Mugwort Fermented Lactic Acid Bacteria"

A plant-based, live probiotic formulation designed to support digestive health, enhance immunity, and improve overall productivity in livestock.

What is "Mugwort Fermented Lactic Acid Bacteria"?

A functional mixed feed containing phyto-derived probiotics (Lactobacillus rhamnosus YL399 and Lactobacillus plantarum YL198) as its key active ingredients.

This formulation is designed for use with: Cattle, Pigs, Chickens and Sheep

Lactobacillus rhamnosus YL399 and Lactobacillus plantarum YL198

How "Mugwort Fermented Lactic Acid Bacteria" Works

When administered daily in the appropriate amount, Mugwort Fermented Lactic Acid Bacteria helps improve and stabilize the intestinal environment by:

Increasing beneficial microorganisms such as lactic acid bacteria and bifidobacteria

  • Suppressing harmful bacteria such as Escherichia coli and Clostridium

These changes contribute to:

  • Enhanced weight gain
  • Improved feed conversion
  • Strengthened immune response

Together, these effects lead to healthier animals and improved overall productivity.

How Works

Key Features of "Mugwort Fermented Lactic Acid Bacteria"

Mugwort Fermented Lactic Acid Bacteria is a plant-derived probiotic not naturally present in livestock intestines. Classified as a lactic acid bacillus, it is large, rod-shaped, and highly resilient. Compared to conventional Lactococcus, it offers:

  • Stronger fermentation activity
  • High resistance to acidic environments, allowing it to survive stomach acid and reach the intestines alive

Each gram contains over 5 billion active cells, which stimulate the production of essential nutrients such as amino acids and vitamins. The YL198 strain colonizes the intestinal lining and forms a protective layer, enhancing the animal’s natural defenses.

Our product has passed rigorous testing, including:

  • Acute and short-term toxicity tests
  • Mycotoxin, heavy metal, cyanuric acid, and melamine screening

It is officially recognized as safe by Japan’s Ministry of Agriculture, Forestry and Fisheries.

High Safety

  • Can be stored at room temperature for over six months
  • Maintains a stable pH of 3.0–3.4 to inhibit unwanted microbial growth
  • Every batch is carefully tested for pH and salinity to ensure consistent quality

Our unique production method allows for lower manufacturing costs compared to animal-based probiotics—making it a more economical option for producers.

Feeding Trial by "Mugwort Fermented Lactic Acid Bacteria"

Feeding Trial Results chickens in JAPAN

Impact on Beneficial and Harmful Gut Bacteria

Trial Summary: Chicks (8 days old) were given 0.5 ml/day of Mugwort Fermented Lactic Acid Bacteria to chicken for one month

Beneficial and Harmfu

Effect of Increased Lactic Acid Bacteria
  • Improved balance of intestinal flora
  • Enhanced digestive function and smoother bowel movements
  • Strengthened immune response
  • Contributed to overall better health and resilience
Effect of Reduced E. coli
  • Suppression of harmful bacteria
  • Reduction in indigestion and diarrhea
  • Restoration of a healthy gut microbiome
  • Improved overall health and condition of chicks

Effects on Weight Gain and Feed Efficiency in chicks

Trial Summary: Chicks (8 days old) were given 0.2 ml/day of Mugwort Fermented Lactic Acid Bacteria for 13 days

Weight Gain and Feed Efficiency

Verified Outcomes
  • >10% increase in average body weight compared to the control group
  • No significant difference in feed intake between groups
  • Feed efficiency improved by 27%, as the supplemented group achieved greater weight gain despite consuming the same amount of feed

Wormwood Fermented Lactic Acid Bacteria Enhance Umami

Supplementation with mugwort fermented lactic acid bacteria has been shown to increase inosinic acid levels—a key compound responsible for the rich umami taste in meat.

Umami Ingredients

Umami Ingredients

Taste Evaluation

Taste Evaluation

Feeding Trial Results chickens in GHANA

Objective

To assess the effects of Mugwort Fermented Lactic Acid Bacteria on growth, feed efficiency, health indicators, and overall condition in laying hens.

Test Parameters
  • Poultry Type : Layers
  • Age : 47 weeks
  • Number of Birds : 20
  • Duration : 30 days
  • Environment : Humid conditions
  • Feed Type : Koudijs commercial product
Methodology
  • Week 1–3 : 2 ml of mugwort supplement per bird daily
  • Week 4–6 : Reduced to 1 ml daily
  • After 6 weeks : Maintenance dose of 0.5 ml per bird
Results
  • Growth Rate : ~5% average weight gain during the test period
  • Feed Conversion Ratio (FCR) : Approximately 2.5 (2.5 units of feed per unit of gain)
  • Mortality : Zero bird deaths
  • Behavioral Improvement : Birds appeared more active and vigorous
Notable Observations
  • Regrowth of feathers in previously withered birds
  • Weak birds became visibly stronger and more energetic
  • Egg production improved by approximately 35%
  • Overall flock health visibly improved
Conclusion

This small-scale trial demonstrates that mugwort supplementation can have a significant positive impact on the health and productivity of laying hens. The results support broader application in commercial poultry operations.

Feeding Trial Results Piglets in JAPAN

Evaluation of Weight Gain and Feed Efficiency

A total of 80 weaned piglets were divided into two groups:

  • Control group : 40 piglets
  • Test group : 40 piglets receiving 10 ml/day of Wormwood Fermented Lactic Acid Bacteria added to their drinking water

After 4 weeks, weight gain and feed efficiency were measured to assess the probiotic’s effectiveness.

Weight Gain and Feed Efficiency

Evaluation of Serum Biochemical Indices

The same test and control groups were also evaluated for changes in serum biochemical parameters after 4 weeks of probiotic supplementation.

Indicators such as total protein, immunoglobulin (IgG), and urea levels were measured to assess internal health and immune response.

Evaluation of Serum Biochemical Indices

Feeding Trial Results cattle in JAPAN

Evaluation of Beneficial and Harmful Gut Bacteria

Fecal samples were collected from two groups of calves:

  • Test group : Calves (8 days old) received 20 ml/day of Mugwort Fermented Lactic Acid Bacteria for 1 month
  • Control group : Calves fed a standard diet without supplementation

The samples were analyzed to assess the growth of beneficial and harmful bacteria, verifying the probiotic’s impact on gut health.

Evaluation of Beneficial and Harmful Gut Bacteria

Evaluation of Milk Yield in dairy cows

A total of 600 dairy cows were divided as follows:

  • 300 cows from low-yielding herds
  • 300 cows from medium-yielding herds

Each cow received 30 ml/day of Mugwort Fermented Lactic Acid Bacteria. Milk production was measured one week after administration, across the period July 8 to October 28, and compared with results from the same period the previous year.

Low-yielding herds

Low-yielding herds

  • Maximum daily yield per cow increased from 20.6 kg to 24.4 kg
  • Average daily yield per cow increased from 14.77 kg to 17.75 kg

→ Average increase: 20%

Medium-yielding herds(Year-on-Year Comparison)

Medium-yielding herds

  • iMaximum daily yield per cow increased from 31.5 kg to 38.2 kg
  • Average daily yield per cow increased from 27.1 kg to 29.3 kg

→ Average increase: 7%

How to use Mugwort Fermented Lactic Acid Bacteria

For large-scale poultry Operation(Adding to feed)

When mixing into a silo for poultry feed, add the product using a feed truck (lorry) at a ratio of. 0.1%–1% of the total feed volume

Dosage guideline : Approximately 0.2 ml per bird per day

*Note : If the feed is stored for an extended period with excess moisture, mold may develop. Please ensure the water content is properly controlled during mixing.

For large-scale poultry Operation

*Feed: Product: Water=10t : 10L : 100L

For milking cows(Adding to concentrated feed)

Give 10 ml per adult cow, twice daily (morning and evening), mixed with feed.

For best results, pre-soak the concentrated feed before feeding.

For milking cows

For calves(Adding to milk)

Mix 10 ml of the product into milk and feed to each calf twice a day—once in the morning and once in the evening.

For calves

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